Journal: Diabetologia

Article Title: The metabolomic signature of weight loss and remission in the Diabetes Remission Clinical Trial (DiRECT)

doi: 10.1007/s00125-023-06019-x

Figure Lengend Snippet: Metabolites associated with intervention from linear model (named/annotated representative features only)

Article Snippet: Glycosyl ceramide (d18:2/24:1, d18:1/24:2) a , Lipid , Hexosylceramides , Metabolon , 0.64 , 0.46 , 0.82 , 5.16×10 −8.

Techniques:

Journal: American Journal of Physiology - Cell Physiology

Article Title: CERS6-derived ceramides aggravate kidney fibrosis by inhibiting PINK1-mediated mitophagy in diabetic kidney disease

doi: 10.1152/ajpcell.00144.2023

Figure Lengend Snippet: Abnormal CER metabolism in the kidney of diabetic mouse models. Body weight ( A ) and blood glucose ( B ) in db/db mice (pink) and db/m mice (black) were measured at the indicated weeks of age. Data are shown as means ± SD, n = 6, ** P < 0.01, *** P < 0.001 between db/db and db/m at the same time point. C : 24 h of albuminuria were measured at 20 wk. Data are shown as means ± SD, n = 6, *** P < 0.001. D : representative images of H&E, PAS, MASSON, and immunohistochemical staining for Collagen I in kidneys ( n = 3). Star indicates tubular atrophy. The black arrow indicates basement membrane thickening. The white arrow indicates collagen deposition. Triangle indicates protein droplets in the cytoplasm of renal tubular epithelium. The bar indicates 50 μm. E : analysis of ceramide synthesis mRNA expression in the kidney cortices of db/db mice and db/m mice. Data are shown as means ± SD, n = 6, ** P < 0.01, *** P < 0.001. F : CER levels in the kidney cortices of db/db mice and db/m mice. Data are shown as means ± SD, n = 3, * P < 0.05, ** P < 0.01, *** P < 0.001. G , H : Western blots and quantification of Collagen I, α-SMA, and Cers6 in the kidney cortices of db/db mice and db/m mice. Data are shown as means ± SD, n = 6, *** P < 0.001. CERS6, ceramide synthase 6; CER, ceramide; H&E, hematoxylin and eosin; PAS, Periodic acid-Schiff.

Article Snippet: To predict the possibility of the combination between CERS6-derived CER and PINK1 protein, we docked CER (d18:1/14:0) ( Fig. 5 A ) and CER (d18:1/16:0) ( Fig. 5 B ) ceramide to the PINK1 domain using Autodock Vina.

Techniques: Immunohistochemical staining, Staining, Membrane, Expressing, Western Blot

Journal: American Journal of Physiology - Cell Physiology

Article Title: CERS6-derived ceramides aggravate kidney fibrosis by inhibiting PINK1-mediated mitophagy in diabetic kidney disease

doi: 10.1152/ajpcell.00144.2023

Figure Lengend Snippet: Knockdown of Cers6 in db/db mice regulated CER metabolism and reduced tubulointerstitial fibrosis. A : analysis of ceramide synthesis mRNA expression in the kidney cortices of db/db + Cers6 shRNA, db/db + vector shRNA, and db/m + vector shRNA. Data are shown as means ± SD, n = 3, * P < 0.05, *** P <0.001 vs. db/m + vector shRNA, ### P < 0.001 vs. db/db + Cers6 shRNA. B , C : Western blots and quantification of Collagen I, α-SMA, and Cers6 in the kidney cortices of each group. Data are shown as means ± SD, n = 3, ** P < 0.01, *** P < 0.001 vs. db/m + vector shRNA, # P < 0.05, ## P < 0.01, ### P < 0.001 vs. db/db + Cers6 shRNA. D : CER levels in the kidney cortices of each group. Data are shown as means ± SD, n = 3, *** P < 0.001 vs. db/m + vector shRNA, ### P < 0.001 vs. db/db + Cers6 shRNA. Body weight ( E ), and blood glucose ( F ) in db/db + Cers6 shRNA (green triangle), db/db + vector shRNA (pink square), and db/m + vector shRNA (black circle) were measured at the indicated weeks of age. Data are shown as means ± SD, n = 3, ** P < 0.01, *** P < 0.001 between db/db + vector shRNA and db/m + vector shRNA at the same time point. G : Twenty-four hours of albuminuria were measured at 20 wk. Data are shown as means ± SD, n = 3, *** P < 0.001 vs. db/m + vector shRNA, ### P < 0.001 vs. db/db + Cers6 shRNA. H : representative images of H&E, PAS, MASSON, and immunohistochemical staining for Collagen I in kidneys. Star indicates tubular atrophy. The black arrow indicates basement membrane thickening. The white arrow indicates collagen deposition. Triangle indicates protein droplets in the cytoplasm of renal tubular epithelium. The bar indicates 50 μm. CERS6, ceramide synthase 6; CER, ceramide; H&E, hematoxylin and eosin; PAS, Periodic acid-Schiff.

Article Snippet: To predict the possibility of the combination between CERS6-derived CER and PINK1 protein, we docked CER (d18:1/14:0) ( Fig. 5 A ) and CER (d18:1/16:0) ( Fig. 5 B ) ceramide to the PINK1 domain using Autodock Vina.

Techniques: Knockdown, Expressing, shRNA, Plasmid Preparation, Western Blot, Immunohistochemical staining, Staining, Membrane